Journal: Oncotarget

Article Title: α2,3-sialyltransferase type I regulates migration and peritoneal dissemination of ovarian cancer cells

doi: 10.18632/oncotarget.15994

Figure Lengend Snippet: (A) Using Oncomine TCGA ovarian cancer genomics (562 ovarian carcinoma samples analyzed on an Affymetrix Human Genome U133 array; 12,624 measured genes), we compared different ST mRNAs, including α2,3-, α2,6-, and α2,8-linked ST, with survival time using a tercile approach. Patients with an upper one-third mRNA expression were defined as the high subgroup, while others with lower two-thirds mRNA expression were defined as the low subgroup. (B-C) IHC analysis of ST3GalI was performed on commercial human ovarian cancer tissue array samples (Super Bio Chips, CJ2, Korea). The intensity scores were as follows: 0, no staining; 1, weak; 2, moderate; 3, strong. Low ST3GalI included weak, moderate or no staining; high ST3GalI was defined as strong staining. Scale bars representing 20μm were added from an image taken at identical magnification and resolution. The percentage was determined in the early stage (FIGO stage I &II) or late stage (FIGO stage III&IV) disease groups. The Fisher's exact test was used to statistically analyze the percentage for the early and late stages. Kaplan-Meier survival curves were used to analyze OS in low- and high-ST3GalIgroups. (D-E) Transwell migration and matrigel invasion of ES2 human ovarian cancer cells with either ST3GalI knocked-down or over-expressed was assayed. Total numbers of cells in 7 random fields were counted. Data shown are the mean ± SD of 3 separate experiments (*: p < 0.05, **: p <0.01.). Data on the Y-axis represented relative value compared to control. Immunoblots were quantified using Image J software.

Article Snippet: IHC specimens (59) from the CJ2 human ovarian cancer tissue array were obtained from a commercial tissue array (Super Bio Chips, Seoul, S. Korea) with complete clinical data, including clinical stage, grade and overall survival (OS).

Techniques: Expressing, Staining, Migration, Control, Western Blot, Software

Journal: PLoS ONE

Article Title: Roles of DPY30 in the Proliferation and Motility of Gastric Cancer Cells

doi: 10.1371/journal.pone.0131863

Figure Lengend Snippet: (A-D) Immunohistochemical staining demonstrated the overexpression of DPY30 in gastric cancer tissues. Notably its overexpression was obviously greater in invading cancer cells (B-D) than in normal gastric mucosa (A). (E) The mRNA level of DPY30 in gastric cancer cells (SNU1, SNU16, SNU216, SNU620, SNU638 and NCI-N87) and normal gastric epithelial cell (HFE145) was determined by real-time PCR using specific primers for DPY30. GAPDH was used to normalize data. Values shown are the means ± SDs of the three independent experiments performed in triplicate. *, p < 0.01 (Student’s t test, versus HFE145). (F) The expression of DPY30 in gastric cancer tissues was examined by real-time PCR using specific primers for DPY30. GAPDH was used to normalize data. Values are the means ± SDs of three independent experiments performed in triplicate. *, p < 0.01; **, p < 0.05 (Student’s t test, versus normal).

Article Snippet: Immunohistochemical staining with rabbit anti-human DPY30 polyclonal antibody (Sigma-Aldrich) was performed on a gastric cancer tissue array (n = 59) purchased from SUPER BIO CHIPS (Seoul) or on 4-μm sections of paraffin-embedded specimens.

Techniques: Immunohistochemical staining, Staining, Over Expression, Real-time Polymerase Chain Reaction, Expressing